Figure 1.

Mycl^GFP expression identifies specified progenitors of pDCs and cDC1s but not cDC2s. (A) Frequency of Mycl-GFP expression among Lin⁻CD11c⁻MHCII⁻CD135⁺CD117^int BM progenitors, gated to indicate populations sorted in B and C. (B) Analysis of sorted progenitor populations after 6 d of culture in Flt3l. (C) Quantification of pDCs (Ly6D⁺SiglecH⁺), CD11c⁺MHCII⁺ cDC1s (CD24⁺CD172a⁻), and cDC2s (CD172a⁺). (D–G) Lin⁻CD135⁺CD11c⁺MHCII^−/int populations of the BM analyzed for Mycl-GFP expression including Ly6D⁺SiglecH⁺ pDCs in E, Ly6D⁻SiglecH⁻CD117^intCD226⁺ pre-cDC1s in F, and Ly6D⁻SiglecH⁻CD117⁻CD115⁺Ly6C⁺ pre-cDC2s in G. Mycl^GFP/+ mice were used for A–C, and Mycl^GFP/GFP mice for D–G. Numbers inside flow cytometry panels quantify the frequency of indicated populations as a percentage of the parent gate. *, P < 0.05; ****, P < 0.0001. All data are representative of at least three independent experiments (n = 5–10).