Figure 2.

The switch from MYC to MYCL expression occurs within the CDP and CLP on pre-cDC1 and pre-pDC specification. (A) Representative pregating scheme for flow cytometry analysis of BM CLPs (Lin⁻CD11c⁻MHCII⁻Ly6C⁻CD11b⁻CD115⁻CD135⁺CD127⁺) from Myc^GFP/GFP and Mycl^GFP/GFP mice. (B and C) Frequency of ALPs (SiglecH⁻Ly6D⁻), BLPs (SiglecH⁻Ly6D⁺), and pre-pDCs (SiglecH⁺Ly6D⁺) within MYC-GFP⁻ and MYC-GFP⁺ CD127⁺ CLPs from Myc^GFP/GFP mice, gated as in A. (B) Representative plot. (C) Data from four samples pooled from two independent experiments. (D) Frequency of ALPs, BLPs, and pre-pDCs within Mycl-GFP^low, Mycl-GFP^int, and Mycl-GFP^high CLPs from Mycl^GFP/GFP mice, as gated in A. (E) Representative pregating scheme for flow cytometry analysis of BM CD115⁺ CDPs (Lin⁻CD11c⁻MHCII⁻CD127⁻CD135⁺CD117^int) from Myc^GFP/GFP and Mycl^GFP/GFP mice. (F and G) Frequency of pre-cDC1s (CD226⁺Ly6D⁻) and pre-pDCs among MYC-GFP⁻ and MYC-GFP⁺ CD115⁺ CDPs. (F) Representative plot. (G) Data from four samples pooled from two independent experiments. (H) Frequency of pre-cDC1s (CD226⁺Ly6D⁻) and pre-pDCs among Mycl-GFP⁻ and Mycl-GFP⁺ CD115⁺CDPs. Numbers inside flow cytometry panels quantify the frequency of indicated populations as a percentage of the parent gate.