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. 2021 Dec 27;219(2):e20211483. doi: 10.1084/jem.20211483

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© 2021 Anderson et al.

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Figure 5. — MYC repression requires Irf8 on specification of DC and monocyte (Mono) progenitors. (A) Flow cytometry comparing gated populations in the BM of Irf8^+/+Myc^GFP/GFP and Irf8^−/−Myc^GFP/GFP mice. (B) Populations gated in A are labeled above, analyzed on the basis of CD117 and MYC-GFP expression for the indicated genotypes, and quantified. (C) Frequency of the populations defined in B on the basis of CD115 and CD135 expression, as labeled. The top panel with filled circles corresponds to CD117⁺MYC-GFP⁺ populations, and the bottom panel with open circles corresponds to the CD117⁻MYC-GFP⁻ population. (D) Analysis of CD117 and MYC-GFP expression in the CLP from BM of the indicated genotypes. (E) As defined in D, the frequency of pre-pDCs within the CLP is analyzed on the basis of Ly6D and SiglecH expression. (F) Expression of CD117, MYC-GFP, analyzed for BM-pDCs. (H) Expression of CD117, MYC-GFG is analyzed for pre-cDC2s. (I) Expression of CD117, MYC-GFG is analyzed for cMoPs and monocytes. Numbers inside flow cytometry panels quantify the frequency of indicated populations as a percentage of the parent gate. All analysis is representative of populations in the BM of mice with the indicated genotypes. Data are representative of two independent experiments (n = 7 or 8).