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. 2021 Dec 27;219(2):e20211483. doi: 10.1084/jem.20211483

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© 2021 Anderson et al.

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Figure 6. — The +41kb Irf8 enhancer is required pDC maturation but not MYC repression on specification. (A) CD117^intCD226⁺ pre-cDC1s. (B) ALP (SiglecH⁻Ly6D⁻), BLP (SiglecH⁻Ly6D⁺), and pre-pDC (SiglecH⁺Ly6D⁺) populations illustrated above, and the same bulk pregate population illustrated on the basis of MYC-GFP and CD117 expression. (C) Bulk BM pDCs illustrated on the basis of CCR9 and MYC-GFP expression above, or maturation markers CCR9 and MHCII below. (D) Analysis of BM pDCs on the basis of Mycl-GFP and CCR9 expression for the indicated genotypes on a Mycl^GFP and Irf8 +41kb^−/− background. (E) Pre-cDC2s illustrated on the basis of Ly6C and MYC-GFP expression above, and CD117 and MYC-GFP expression below. Flow cytometry of BM from mice with the indicated genotypes on the Myc^GFP/GFP background. All populations quantified as percent frequency of the parent population defined by the indicated pregate. Data are representative of two independent experiments (n = 6). (F) Mycl-GFP expression in bulk BM pDCs as defined in D for the indicated genotypes.