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. 2021 Dec 27;219(2):e20211483. doi: 10.1084/jem.20211483

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© 2021 Anderson et al.

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Figure 7. — MYCL expression is maintained in proliferating pre-pDCs and elevated on terminal differentiation in the BM. (A) Frequency of M phase cycling cells (hGeminin-Venus⁺) among bulk CC9⁺ and CCR9⁻ BM pDCs. (B and C) Representative gating scheme for flow cytometry analysis of lymphoid progenitor populations, pre-pDCs, immature, and mature BM pDCs in Fucci2⁺Mycl^gfp/gfp mice. (D) Fucci2 cell cycle analysis on the basis of hGeminin-Venus and hCDT-mCherry expression for the indicated populations, defined in B and C. (E and F) Quantification of the frequency of hGeminin-Venus/YFP⁺ and hCDT-mCherry^high for the indicated populations. (G) Mycl-GFP and CCR9 expression analyzed for the populations indicated above in D. (H) Mycl-GFP expression for the populations indicated above in D. Numbers indicate MFI. (D) Analysis of hCDT-mCherry and Mycl-GFP expression for the populations indicated in D in Fucci2 hemizygous Mycl^GFP/+ mice. All data are representative of two independent experiments (n = 4). Populations were defined as follows: ALP defined as Lin⁻CD135⁺CD11c⁻MHCII⁻Ly6C⁻CD115⁻CD127⁺CD117^int-lowLy6D⁻SiglecH⁻, pre-pDC (A) defined as Lin⁻CD135⁺CD11c⁻MHCII⁻Ly6C⁻CD115⁻CD127⁺CD117^int-lowLy6D⁺SiglecH⁺, pre-pDC (B) defined as Lin⁻CD135⁺CD11c⁺CD115⁻Ly6D⁺SiglecH⁺CCR9⁻MHCII⁻, immature (Imm) pDC defined as Lin⁻CD135⁺CD11c⁺CD115⁻Ly6D⁺SiglecH⁺CCR9⁺MHCII⁻, and mature (Mat) pDC defined as Lin⁻CD135⁺CD11c⁺CD115⁻Ly6D⁺SiglecH⁺CCR9⁺MHCII⁺. Data are representative of two independent experiments (n = 4). Norm, normal.