Figure S5.

Reduced cell cycling and high Mycl-GFP expression correlate with commitment of pre-cDC1s to immature cDC1s. (A) Representative gating scheme for flow cytometry analysis of cDC1s and cDC1 precursors in the BM of Fucci2⁺Mycl^gfp/gfp mice. (B and C) Cell cycle analysis on the basis of Geminin-Venus and CDT-mCherry expression, quantified as illustrated in Fig. 2. (D) Mycl-GFP expression for the indicated populations. Histograms are color-coded and correspond to gated populations as defined in A. Numbers inside flow cytometry panels quantify the frequency of indicated populations as a percentage of the parent gate. All data are representative of two independent experiments (n = 4). Pre-cDC1 (A), labeled in orange, defined as Lin⁻Ly6D⁻SiglecH⁻CD135⁺CD115⁺CD117^intCD226⁺; pre-cDC1 (B), labeled in blue, defined as Lin⁻Ly6D⁻SiglecH⁻CD135⁺CD115⁻CD117^intCD226⁺CD11c⁺MHCII^int; immature (Imm) cDC1, labeled in red, defined as Lin⁻Ly6D⁻SiglecH⁻CD135⁺CD115⁻CD117^intCD226⁺CD11c^highMHCII^high.