Fig. 7. Adoptive transfer of BMPR1α-deficient CD4⁺CD45RB^high cells induces more severe colitis.

(A to F) TCRα⁻ recipient mice received a transfer of either WT or BMPR1α^T− CD4⁺CD45RB^high cells. (A to C) Weight loss, bleeding and diarrhea were scored over time. (D) Total colon length was assessed on day 21. (E) Histological analysis of colonic cross-sections by hematoxylin and eosin staining was performed on the indicated mice on day 21. Scale bars equal 100 μM. (F) Flow cytometry analysis of cytokines and Rorc in CD4⁺ T cells isolated from the colons of recipient mice. (G) qRT-PCR analysis of IL-6, IL-1β, IL-17, Rorc and IFN-γ transcripts in CD4⁺ T cells isolated by flow cytometry sorting from colons of recipient mice. All results are representative of four mice per group analyzed in three independent experiments. Quantified data are means ± SD from all experiments. *P< 0.05, **P< 0.01, ***P< 0.001, ****p< 0.0001, as determined by Student’s t-test.