Mn-induced YY1 phosphorylation increases interaction between YY1 and HDACs in H4 astrocytes. (A) Astrocytes were pre-treated with Hesp (250 nM, 1 h), then exposed to Mn (250 μM, 3 h) and immunoprecipitated for YY1, followed by western blotting for HDAC1. (B) Astrocytes were pre-treated with CX (10 μM, 1 h), then exposed to Mn (250 μM, 3 h) and co-immunoprecipitated for YY1/HDAC3 interaction. (*p < 0.05, **p < 0.01, ***p < 0.001, compared to the control; #p < 0.05, ANOVA followed by Tukey’s post hoc test; N = 3).