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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: J Med Chem. 2021 Aug 18;64(17):12572–12581. doi: 10.1021/acs.jmedchem.1c00395

Figure 3.

Figure 3.

Analysis of LGR4 and LGR5 expression and R4Fu binding in cancer cell lines. (A). WB of LGR4 in recombinant and cancer cell lines. Actin is loading control. (B) WB of LGR5 in HEK293T and cancer cell lines. GAPDH is loading control. (C) Binding and internalization of peptibody and PDC in LoVo and LoVo-4KO cells by fluorescence confocal microscopy. Cell were incubated with the ligands at 36 nM, fixed, permeabilized, and stained with Alexa-488-labeled anti-human IgG (green). Nuclei were counter-stained with To-PRO3 (blue).