Fig. 6.

GFAP-Cre expression specifically deletes C3 from astrocytes in EAE PID16 optic nerves. a Representative images of tdTomato expression in the optic nerve of C3^tdT–fl x GFAP-Cre mice at EAE PID16; Scale bar = 50 μm. b Quantification of tdTomato expression in the optic nerve of C3^tdT–fl x GFAP-Cre mice at EAE PID16, presented as MFI; AU, arbitrary unit. c Representative images of tdTomato expression in SOX9⁺ astrocytes in the optic nerve of C3^tdT-–fl x GFAP-Cre at EAE PID16. d The percentage of tdTomato⁺ SOX9⁺ astrocytes in the optic nerve of C3^tdT–fl x GFAP-Cre mice at EAE PID16, quantified by IHC. e Representative images of tdTomato expression in IBA1 + cells in the optic nerve of C3^tdT–fl x GFAP-Cre mice at EAE PID16; Scale bar = 20 μm. f The percentage of tdTomato⁺/IBA1⁺ cells in the optic nerve of C3^tdT–fl x GFAP-Cre mice at EAE PID16, quantified by IHC. Significance between groups was assessed by unpaired t-test. Error bars represent SEM