Table 1. NS physical properties.

P4, TSA, and SAHA NS formulations and sizes. Particles were formulated via wet milling (10 hours) in Pluronic F127. Size, PDI, and ζ-potential are represented as means ± SD (n ≥ 3).

Drug concentration	% F127 (w/v)	Beads type-mass (g)	Tube size (ml)	Size (nm)	PDI	ζ-Potential (mV)
P4 (5 mg/ml)	2	ZROB05 (1.5)	2.0	346.4 ± 15.8	0.11 ± 0.07	−1.08 ± 0.84
P4 (25 mg/ml)	2	ZROB05 (2.4)	2.0	302.5 ± 9.7	0.17 ± 0.02	−1.59 ± 0.21
P4 (50 mg/ml)	2	ZROB05 (2.4)	2.0	305.9 ± 5.8	0.21 ± 0.15	−0.087 ± 0.012
P4 (80 mg/ml)	2	ZROB05 (2.4)	2.0	376.7 ± 14.1	0.19 ± 0.12	−0.92 ± 0.72
P4 (250 mg/ml)	6	ZROB05 (3.5)	2.0	315.5 ± 7.9	0.17 ± 0.06	−1.48 ± 0.17
TSA (1.5 mg/ml)	4	ZSB05 (1.5)	1.5	215.9 ± 12.9	0.12 ± 0.07	−0.06 ± 0.04
SAHA (1.5 mg/ml)	4	ZROB05 (1.5)	1.5	263.5 ± 48.6	0.37 ± 0.24	−2.44 ± 0.89