Fig. 4.

Three methods for RT-LAMP result readout by PaGeR platform. (A) The MG colorimetric detection method for RT-LAMP. (B) The calcein-based fluorometric detection method for RT-LAMP (within the yellow dashed line). (C) Visual lateral dipstick biosensor detection. All three ways could be detected by the PaGeR (highlighted with green dashed line). The sensitivity tests of colorimetric and fluorometric detection were highlighted with red dashed line. (D) The intensity for 10-fold serial diluted plasmid by colorimetric detection. (E) The fluorescence intensity with 10-fold serial diluted plasmid by calcein-based fluorometric detection. Data were averages of three independent experiments, each consisting of three technical replicates. Data were shown as the mean ± SD. **p < 0.01 (Student's t-test). C, control; P, positive. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)