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Analysis of the CCR5 alleles detected in 13 clonal lines. Individual clones were isolated from cell populations transduced with both sgRNA1 and sgRNA2. The 558 bp PCR product for each allele was amplified and sequenced. (A) The clones with one mutant allele and one wild-type sequence. (B) The clones with two mutant alleles, one FS mutation, and one IF mutation. (C) The clones carrying two FS mutant alleles. FS, frameshift; IF, in-frame.
