FIGURE 8.

Cav1‐knockdown mice displayed reduced BBB permeability and lighter brain edema after t‐MCAO. (A) Six‐week‐old mice received intracerebroventricular injections of control virus (AAV9‐mCherry, two microliters of 1013 vg/ml) or one that decreased expression of Cav1(AAV9‐mCherry‐Cav1). Three weeks after injection, distribution of AAV9‐mCherry on brain sections were examined under confocal microscope. Red fluorescence was clearly seen in brain parenchyma. Comparison of mRNA levels (B) and protein levels (C, D) in cortical lysate from animals injected with either AAV9‐control or AAV9‐Cav1 showed significant decreases in Cav1 expression. *p < 0.05, **p < 0.0 vs. the AAV9‐control group. (E, F) Representative images and quantitative analysis of and EB extravasation at 6h after t‐MCAO between the AAV9‐control and AAV9‐Cav1 group. (G‐I) Representative T2‐weighted images and comparison of brain edema index in different groups. *p < 0.05, **p < 0.01 vs. the AAV9‐control group