FIGURE 9.

GCX degradation‐induced BBB disruption is Cav1‐dependent. (A) Histological analysis of 40 KD FITCDextran (green) leakage from isolectin‐B4 (IB4)‐stained blood vessels (red) in Cav1 knockdown mice at 6h after GCX degrasation induced by HAase. Upper and lower histological panels displayed BBB leakage in the cortex (Scale bar, 20 μm) and hippocampus (Scale bar, 50 μm), respectively. (B, C) EM evaluation of vesicles‐transport function showed that Cav1‐knockdown significantly reduced the the number of HRP‐filled vesicles in HAase treated mice, compared with the control group. **p < 0.01 vs. the AAV9‐control group. (D, E) Western blot results of p‐Cav1, p‐syndecan1. *p < 0.05 and ***p < 0.001vs. the sham group. (F) The association of syndecan1 and Src detected by immunoprecipitation at 6h after using HAase or t‐MCAO. (G) The direct interaction between syndecan1 and Src detected by GST pull down