Subretinal injection of chimeric HDAds in rat eyes. Representative brightfield (A, D, G) and eGFP fluorescent (C, F, I) fundus photographs and corresponding in vivo OCT line scans (B, E, H; denoted by bright green arrows in A, D, G) are shown for transscleral subretinal injections of control buffer (A–C), HDAd5/3-CMVp-eGFP (D–F) and HDAd5/35-CMVp-eGFP (G–I). Injection sites are denoted by white arrowheads. Scale bars = 100 μm in (B), (E), and (H). Abbreviations of retinal layers: Vit, GCL, INL, ONL, RPE, and CH. CH, Choroid; OCT, optical coherence tomography; RPE, retinal pigmented epithelium; HDAds, helper-dependent adenoviruses; Vit, Vitreous.