FIG. 4.

Comparison of kinetic parameters of [PSI⁺] curing by Hsp104 overproduction, Hsp104 inactivation, and GuHCl treatment. Yeast strains were GT81-1C (A), OT55 (B), and OT56 (C). Curing conditions included induction of GAL-HSP104 (Hsp104), induction of GAL-HSP104-KT (Hsp104-KT), growth in the presence of 5 mM GuHCl in rich YPD medium (YPD + 5 mM GuHCl) or in synthetic Gal+Raf medium (Gal+Raf + 5 mM GuHCl), and deletion of the HSP104 gene (Hsp104 Deletion). Designations are shown in the box in panel A. See Materials and Methods for procedures. Data for hsp104Δ are from Table 2. These results were obtained by dissecting the diploid GT84, which is isogenic to GT81-1C.