Table 3.

Effect of eggshell temperature (EST) during late incubation [≥E17–E19.5; 37.8°C (control) or 36.7°C (lower)] and/or feeding strategy after hatch [immediate access to feed and water (early) or 51–54-h deprivation (delayed)] on coccidiosis of broilers at d 0, 6, 7, or 14 post Eimeria inoculation (d 21 post hatch) to induce necrotic enteritis (mean ± SE).

	N c	Coccidiosis severity (TMLSd 0–12)				Coccidiosis incidencee (%)
		Day post Eimeria inoculationf				Day post Eimeria inoculationf
		0	6	7	14	0	6	7	14
EST
Control	16	2.2 ± 0.18	3.8 ± 0.24	2.8 ± 0.20	0.9 ± 0.10a	85.3 ± 4.17	92.1 ± 3.09	92.1 ± 3.09	70.0 ± 5.1
Lower	16	1.9 ± 0.21	4.1 ± 0.23	3.2 ± 0.21	0.6 ± 0.07b	90.6 ± 5.15	96.1 ± 2.21	96.0 ± 2.29	54.3 ± 5.53
Feeding strategy
Early	16	2.2 ± 0.23	3.8 ± 0.22	3.0 ± 0.20	0.8 ± 0.10	86.0 ± 5.28	94.8 ± 2.53	94.8 ± 2.53	55.6 ± 5.52
Delayed	16	2.0 ± 0.17	4.0 ± 0.25	3.0 ± 0.21	0.9 ± 0.08	87.7 ± 4.13	93.4 ± 2.84	93.2 ± 2.96	68.8 ± 5.18
EST × Feeding strategy
Control × Early	8	2.4 ± 0.26	3.7 ± 0.33	2.8 ± 0.30	1.0 ± 0.16	86.1 ± 5.76	94.7 ± 3.62	92.1 ± 4.37	67.5 ± 7.41
Control × Delayed	8	2.1 ± 0.24	3.8 ± 0.35	2.9 ± 0.26	1.0 ± 0.12	84.4 ± 6.02	89.5 ± 4.98	92.1 ± 4.37	72.5 ± 7.06
Lower × Early	8	1.6 ± 0.40	4.0 ± 0.30	3.2 ± 0.26	0.5 ± 0.10	85.7 ± 13.23	94.9 ± 3.53	97.4 ± 2.53	43.9 ± 7.75
Lower × Delayed	8	2.0 ± 0.24	4.1 ± 0.36	3.1 ± 0.32	0.8 ± 0.10	92.0 ± 5.43	97.4 ± 2.60	94.3 ± 3.92	65.0 ± 7.54
P -values
EST		0.23	0.68	0.34	0.01	0.79	0.46	0.35	0.09
Feeding strategy		0.87	0.79	0.99	0.38	0.58	0.92	0.59	0.20
EST × Feeding strategy		0.34	0.85	0.53	0.30	0.80	0.61	0.57	0.38
Sex		0.31	0.57	0.90	0.39	0.77	0.84	0.27	0.54

^a,bMeans within a column and factor lacking a common superscript differ (P < 0.05).

^cNumber of pens.

^dTMLS = Total mean lesion score. Sum of coccidiosis score for E. acervulina, E. maxima, and E. tenella.

^eBroilers with a TMLS of 1 or more were classified as coccidiosis positive.

^fIn total, 100, 153, 150, and 161 broilers for d 0, 6, 7, and 14, respectively.