Table 4.

Effect of eggshell temperature (EST) during late incubation [≥embryonic days 17–19.5; 37.8°C (control) or 36.7°C (lower)] and/or feeding strategy after hatch [immediate access to feed and water (early) or 51–54-h deprivation (delayed)] on dysbacteriosis of broilers at d 0 or 14 post Eimeria inoculation (d 21 post hatch) to induce necrotic enteritis (mean ± SE).

	N a	Dysbacteriosis severity (score 0–10)		Dysbacteriosis incidenceb (%)
		Day post Eimeria inoculationc		Day post Eimeria inoculationc
		0	14	0	14
EST
Control	16	2.9 ± 0.15	2.8 ± 0.15	33.8 ± 5.80	33.8 ± 5.29
Lower	16	2.8 ± 0.19	2.9 ± 0.14	21.9 ± 7.31	35.8 ± 5.33
Feeding strategy
Early	16	2.7 ± 0.19	2.9 ± 0.15	25.6 ± 6.65	35.8 ± 5.33
Delayed	16	3.0 ± 0.14	2.9 ± 0.14	33.3 ± 6.33	33.8 ± 5.29
EST × Feeding strategy
Control × Early	8	2.7 ± 0.21	2.9 ± 0.22	27.8 ± 7.47	37.5 ± 7.65
Control × Delayed	8	3.2 ± 0.19	2.8 ± 0.21	40.6 ± 8.86	30.0 ± 7.25
Lower × Early	8	2.7 ± 0.39	2.8 ± 0.21	14.3 ± 13.23	34.1 ± 7.41
Lower × Delayed	8	2.8 ± 0.21	3.0 ± 0.19	24.0 ± 8.54	37.5 ± 7.65
P -values
EST		0.44	0.40	0.88	0.98
Feeding strategy		0.25	0.30	0.94	0.66
EST × Feeding strategy		0.91	0.34	0.44	0.53
Sex		0.95	0.95	0.63	0.89

^aNumber of pens.

^bBroilers with a score of 3 or more were classified as dysbacteriosis positive.

^cIn total, 100 and 161 broilers for d 0 and 14, respectively.