In vivo measurement of cortical dHb, HbO, StO2, oxCCO, reCCO and totCCO under varying FiO2 levels in a group of control mice. Absolute concentrations are extracted from the raw attenuation spectra using NIR-AQUA. Each black dot represents the average value extracted from all the spectra collected at the corresponding FiO2 level in each mouse (mean ± SD, n = 6). Statistical analysis was performed to compare these values under the different FiO2 using one-way ANOVA with repeated measures and a Bonferroni post-hoc test (* - p ≤ 0.05, ** - p ≤ 0.01, *** - p ≤ 0.001). Significance between repeated FiO2 levels is not shown for clarity.