Fig. 1.

Intravital two-photon imaging of the liver of diabetic NAFLD mouse model. (a) Schematic of custom-built video-rate laser scanning two-photon microscopy system. (b) Experimental scheme for induction of diabetic mouse model with NAFLD and intravital liver imaging. (c-d) Body weight, fasting blood glucose of STZ-treated high-fat diet fed mouse (STZ + HFD) and normal diet fed mouse (ND). (e) Representative 3-dimensionally reconstructed image of hepatic lipid droplets (LDs, red) and collagen (green). Scale bars, 100µm. Data are presented as mean ± SD of mean. Statistical significance was set at p-value less than 0.05.