Fig. 4.

Rich phase behavior of the system depends on stoichiometry of RGG-RGG and GST-GFP-RGG. (A) Classification system for the morphologies formed by mixing different ratios of RGG-RGG to GST-GFP-RGG. (B) Representative images of condensates formed across concentrations of RGG-RGG and GST-GFP-RGG tested, depicting a range of phase behaviors. (C) Application of classification system to the assemblies observed in the phase diagram, including film, partial engulfment, and multiphasic complexes. (D) Representative condensates viewed with 3D renderings depicting (Left) film, (Middle) low-coverage partial engulfment, and (Right) high-coverage partial engulfment. (E) Dual-color imaging of multiphasic system incorporating rhodamine B to confirm presence of RGG-RGG in engulfed phase. (Scale bars, 5 μm.)