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. 2021 Dec 15;118(51):e2112625118. doi: 10.1073/pnas.2112625118

Fig. 1.

Fig. 1.

Deletion of endothelial MEKK3 in adult mice induces pulmonary and systemic hypertension. (A) Representative Hematoxylin and Eosin (H&E) staining of Ctrl and Mekk3iECKO mice hearts at 4 wk after tamoxifen injection. Arrowheads point to the right ventricle hypertrophy. (Scale bar, 1,000 μm.) (B–D) Time-course analysis of RV, LV, and septum (S) mass for Ctrl and Mekk3iECKO mice at indicated time points, n = 5 mice at 1 wk and n = 4 mice at 2, with 4 and 8 wk per time point and per group; all mice are male. (E) RVSP of male and female Ctrl and Mekk3iECKO mice at 4 wk after tamoxifen injection, showing no difference between male and female. n = 6 mice for per genotype. (F) Time-course analysis of RVSP for Ctrl and Mekk3iECKO mice at indicated time points, n = 5 mice (3 male and 2 female) per time point and per group. (G–I) Time-course analysis of LV systolic pressure (LVSP), Systolic blood pressure (SBP), and Diastolic blood pressure (DBP) from Ctrl and Mekk3iECKO mice at indicated time points, n = 5 male mice per time point and per group. Bars represent the mean. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns: not significant, calculated by two-way ANOVA with Tukey’s multiple comparison tests.