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. 2021 Dec 15;118(51):e2112625118. doi: 10.1073/pnas.2112625118

Fig. 7.

Fig. 7.

Genetic deletion of endothelial TGFβR1 attenuates MEKK3 deficiency-induced hypertension. (A and B) Right ventricle systolic pressure (RVSP) and RV mass of Ctrl (n = 6, 3 male and 3 female), Mekk3iECKO (n = 8, 4 male and 4 female), Tgfbr1iECKO (n = 6, 3 male and 3 female), and DKO mice (n = 6, 3 male and 3 female) at 3 wk after tamoxifen injection. (C) Representative immunostaining of SMA of Ctrl and Mekk3iECKO, Tgfbr1iECKO, and DKO entire lungs. (Scale bar, 1 mm.) (D) Immunostaining of p-Smad3 in Ctrl and Mekk3iECKO, Tgfbr1iECKO, and DKO lungs. (Scale bar, 50 μm.) (E–H) Left ventricle systolic pressure (LVSP), LV mass, Systolic blood pressure (SBP), and Diastolic blood pressure (DBP) of Ctrl, Mekk3iECKO, Tgfbr1iECKO, and DKO mice at 8 wk after tamoxifen injection. (I) Representative Masson Trichrome staining (up) and SMA staining (down) of Ctrl, Mekk3iECKO, and DKO mice femoral artery sections. (Scale bar, 100 μm [Upper] and 50 μm [Lower].) (J) Quantification of femoral artery diameter from Trichrome-stained sections (n = 30 sections from three pairs of mice per group). (E–H) n = 5 mice (3 male and 2 female) for each group. Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, calculated by one-way ANOVA with Tukey’s multiple comparison tests.