Fig. 1.

Phenotypes of mutant plants deficient in Trx-like proteins. (A) Visible phenotypes of plants deficient in Trx-like proteins. Plants were grown for 4 wk. (B) Confirmation of the knockout of Trx-like proteins by Western blotting. Arrows indicate the proteins of interest. Bands without arrows are nonspecific proteins. Leaf extract from the ACHT1 overexpressor was used as a positive control. Coomassie brilliant blue (CBB)-stained Rubisco large subunit (RbcL) is shown as a loading control. (C) Physiological parameters of plants deficient in Trx-like proteins. Each value is presented as the mean ± SD (n = 5). Different letters indicate significant differences among plant lines (P < 0.05; one-way ANOVA and Tukey honestly significant difference [HSD]).