Fig. 2.

Oxidation of Trx-targeted proteins in Trx-like protein-deficient plants during light-to-dark transitions. (A) Western blotting. The redox states of Trx-targeted proteins in dark-adapted (DA) plants or plants during light-to-dark transitions were monitored. Abbreviations: Ox, oxidized; Red, reduced; RI, redox-insensitive splicing variant. (B) Redox state dynamics of Trx-targeted proteins. Using the data from A, each band intensity was measured, and the redox state was determined and plotted. Black and white bars represent 0- and 700-μmol photons m⁻²·s⁻¹ light intensity, respectively. Each value is presented as the mean ± SD [n = 6 (Col-0) or n = 3 (the others)]. Different letters at each time point indicate significant differences among plant lines (P < 0.05; one-way ANOVA and Tukey HSD).