. Author manuscript; available in PMC: 2022 Aug 1.

Published in final edited form as: J Pathol. 2021 Jun 14;254(5):589–605. doi: 10.1002/path.5704

Table 4.

Characterization of acetylcholine and sodium nitroprusside induced vasorelaxation

	Normal	DMD
Acetylcholine
Control (normal=15, DMD=16)
Maximum relaxation, %	95.59 ± 3.75^α	75.84 ± 4.89^*
EC50, Log M	−8.01 ± 0.06	−7.69 ± 0.15
Denuded (normal=6, DMD=6)
Maximum relaxation, %	4.06 ± 2.99^α	2.59 ± 0.96^{^}
EC50, Log M	NA	NA
L-NAME (normal=6, DMD=6)
Maximum relaxation, %	80.79 ± 3.39^α	57.84 ± 6.52^*
EC50, Log M	−7.30 ± 0.07^#	−7.51 ± 0.13
L-NAME + Indomethacin (normal=6, DMD=6)
Maximum relaxation, %	49.91 ± 5.66^α	35.02 ± 7.28^#
EC50, Log M	−7.41 ± 0.10^#	−7.67 ± 0.12
Sodium nitroprusside
Control (normal=15, DMD=14 for maximum relaxation, DMD=16 for EC50)
Maximum relaxation, %	93.29 ± 2.49^β	102.28 ± 1.56^*
EC50, Log M	−6.69 ± 0.09^β	−7.28 ± 0.14^*
Denuded (normal=6, DMD=6)
Maximum relaxation, %	103.93 ± 2.28	105.18 ± 2.11
EC50, Log M	−7.18 ± 0.11	−7.46 ± 0.20
L-NAME (normal=6, DMD=6)
Maximum relaxation, %	104.73 ± 1.09	104.16 ± 1.07
EC50, Log M	−7.27 ± 0.07	−7.63 ± 0.10^*
L-NAME + Indomethacin (normal=6, DMD=6)
Maximum relaxation, %	103.57 ± 1.62	103.47 ± 1.31
EC50, Log M	−7.45 ± 0.07	−7.66 ± 0.20

Mean ± SEM

EC50, Half-maximal effective concentration

L-NAME, Nω-Nitro-L-arginine methyl ester hydrochloride

Significantly different from the normal group in the same row.

^{^}

For acetylcholine study, significantly different from other three groups in the same column.

For acetylcholine study, significantly different from the control group in the same column.

^α

For acetylcholine study, significantly different from each other in the same column.

^β

For sodium nitroprusside study, significantly different from other three groups in the same column.