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. 2021 Dec 14;12:775680. doi: 10.3389/fphar.2021.775680

FIGURE 1.

FIGURE 1

Bru inhibited GBM cells proliferation in vivo and in vitro. (A) The proliferation-suppressive effect of Bru was determined by cell viability assay. As Bru concentration increased, the viability of three GBM cell lines A172 cells [ (A), left], U251 cells [ (A), middle], and U87 cells [ (A), right] significantly decreased. (B) The inhibitory effect of Bru on three GBM cell lines increased significantly over time, which was determined by cell viability assay. A172 cells [(B), left, 50 nM], U251 cells [(B), middle, 75 nM], and U87 cells [(B), right, 50 nM] were treated with Bru for 12–72 h and then subjected to cell viability assay. (C) The clone formation assay of three GBM cell lines. Bru treatment resulted in the inhibition of cell colonies. (D) Bru treatment (2 mg/kg, daily by oral gavage) inhibited the growth of subcutaneously transplanted U87 cell tumors in nude mice. Representative images of xenograft tumors from nude mice were shown in Panel (D). (E) Assessment of the tumor growth curves in nude mice after Bru treatment. (F) The weight of xenograft tumors from nude mice were shown in Panel (F). *p < 0.05, **p < 0.01, ***p < 0.001 vs, 0 nM group or Control group.