Skip to main content
. 2021 Dec 14;12:775680. doi: 10.3389/fphar.2021.775680

FIGURE 4.

FIGURE 4

ECM1 silencing inhibited proliferation, migration and invasion of GBM cells. (A) The effect of ECM1 knockdown via siRNA silencing. Western blot analysis was used to measure the expression level of ECM1 in GBM cell lines transfected with siRNA for 24–48 h. (B) ECM1 knockdown via siRNA silencing inhibited the proliferation of A172 (left), U251 (middle), and U87 (right) cells. The cells were transfected with siControl or siECM1 for 12–60 h and then subjected to cell viability assay by CCK-8. (C) ECM1 knockdown suppressed the migration abilities of GBM cells. As described, the cells were subjected to cell migration assay by wound healing assay. The representative images for GBM cells were shown in Panel (C) (left), and data from three independent experiments were showed in the histogram (right). (D) ECM1 knockdown suppressed the invasive abilities of GBM cells. The cells were transfected with siControl or siECM1 for 48 h and then subjected to cell invasion assay. The representative images for GBM cell lines were shown in Panel (D) (left), and data from three independent experiments were shown in the histogram (right). (E) GBM cell lines were transfected with siECM1 for 24h, subsequently treated with Bru for 48–96h, and then subjected to cell proliferation assay by CCK-8. *p < 0.05, **p < 0.01, ***p < 0.001 vs., siControl group. & p < 0.05, && p < 0.01, &&& p < 0.001 vs., siControl + Bru group.