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. 2021 Dec 15;118(51):e2110641118. doi: 10.1073/pnas.2110641118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 1. — RNAi screen for OtopL genes required for aversion of acid taste. PER assays were performed by touching the proboscis with either 100 mM sucrose alone or sucrose plus the indicated concentrations of HCl (mM) or organic acids (%). The pH values are indicated in each panel. A full extension is scored as 1.0, a partial extension as 0.5, and no extension as 0. All RNAi lines (B–F) were generated by crossing the indicated UAS-RNAi lines to elav-GAL4;UAS-Dcr2 flies. (A) Effects of removing olfactory organs on the PER responses to the indicated concentrations of HCl. Control (w¹¹¹⁸) and w¹¹¹⁸ flies in which the antenna and maxillary palp were surgically removed (no antenna, no palp). n = 26. (B–F) Effects of RNAi knockdown of different OtopL genes on the PER responses to the indicated acids. (B) Effect of knockdown of OtopLA on the PER to sucrose plus HCl. OtopLA-RNAi1 is UAS-OtopLA-RNAi1 (v104973) crossed to elav-GAL4;UAS-Dcr2 flies (n = 30). The UAS control (n = 26) and GAL4 control (n = 25) were generating by crossing w¹¹¹⁸ to either UAS-OtopLA-RNAi1 or elav-GAL4;UAS-Dcr2, respectively. The blue and green asterisks indicate statistically significance differences between OtopLA silenced flies and the UAS and GAL4 controls, respectively. (C) Effect of knockdown of OtopLB on the PER to sucrose plus HCl. OtopLB-RNAi1 is UAS-OtopLB-RNAi1 (v101936) crossed to elav-GAL4;UAS-Dcr2 flies (n = 27). UAS control (n = 28). (D) Effect of knockdown of OtopLC on the PER to sucrose plus HCl. OtopLC-RNAi1 is UAS-OtopLC RNAi (v108591) crossed to elav-GAL4;UAS-Dcr2 flies (n = 29). UAS control (n = 29). (E) Effect of knockdown of OtopL genes on PERs using the indicated concentrations of tartaric acid. OtopLA-RNAi1 (n = 27), OtopLB-RNAi1 (n = 29), and OtopLC-RNAi1 (n = 28). The “control” is elav-GAL4;UAS-Dcr2 flies (n = 65). The blue asterisks indicate significance differences from the control. (F) Effect of knockdown of OtopL genes on PERs using the indicated concentrations of glycolic acid. OtopLA-RNAi1 (n = 27), OtopLB-RNAi1 (n = 29), OtopLC-RNAi1 (n = 28). The “control” is elav-GAL4;UAS-Dcr2 (n = 65). The blue asterisks indicate significance differences from the control. Mann–Whitney u tests. Error bars, SEMs. *P < 0.05, **P < 0.01, and ***P < 0.001.