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. 2021 Dec 15;118(51):e2110641118. doi: 10.1073/pnas.2110641118

Fig. 2.

Fig. 2.

Mutation of OtopLA impairs gustatory repulsion to acids. (A–F) PER indexes obtained using control (w1118) and OtopLA1 flies upon stimulation of the labellum with 100 mM sucrose alone or 100 mM sucrose mixed with the indicated concentrations of acids. (A) Sucrose and HCl. Control, n = 64. OtopLA1, n = 65. (B) Sucrose and tartaric acid. Control, n = 39. OtopLA1, n = 25. (C) Sucrose and glycolic acid. Control, n = 39. OtopLA1, n = 25. (D) Sucrose and citric acid. Control, n = 39. OtopLA1, n = 25. (E) Sucrose and acetic acid. Control, n = 30. OtopLA1, n = 29. (F) Sucrose and proprionic acid. Control, n = 30. OtopLA1, n = 29. (G–I) Testing for rescue of the OtopLA1 defects in acid aversion by expressing the OtopLAp transgene under control of the LexA knocked into OtopLA1. The PERs were assayed from the following lines using the indicated concentrations of acids: 1) OtopLA1, 2) lexAop-OtopLA, and 3) OtopLA1;lexAop-OtopLA (rescue). Asterisks indicate significance differences between OtopLA1 and the rescue flies. (G) HCl. OtopLA1, n = 30. lexAop-OtopLA, n = 27. OtopLA1;lexAop-OtopLA, n = 29. (H) Tartaric acid. OtopLA1, n = 28. lexAop-OtopLA, n = 26. OtopLA1;lexAop-OtopLA, n = 28. (I) Glycolic acid. OtopLA1, n = 28. lexAop-OtopLA, n = 26. OtopLA1;lexAop-OtopLA, n = 28. (J) Testing whether the OtopLA1 mutation impairs the PER response to sucrose. Control (w1118), n = 27. OtopLA1, n = 29. (K) Testing whether the OtopLA1 mutation impairs the PER response to denatonium mixed with 100 mM sucrose. Control (w1118), n = 27. OtopLA1, n = 29. (L) Testing whether the OtopLA1 mutation impairs the PER response to NaCl mixed with 100 mM sucrose. Control (w1118), n = 27. OtopLA1, n = 29. Mann–Whitney U tests. Error bars, SEMs. *P < 0.05, **P < 0.01, and ***P < 0.001.