Fig. 1.

Deposition and imaging workflow for native ES-IBD/LEEH. (A) Scheme of the native ES-IBD process, proceeding from solution through the gas phase to soft-landing deposition. Before deposition, the beam is mass selected to ensure that only the relevant species land on the surface. (B) Mass spectrum of the Herceptin deposition beam after mass selection. The low charge states indicate that the proteins are in native-like conformations. (C) Scheme of the LEEH measuring and reconstruction process.