Figure 4.
Focal chemical stimulation of the dorsal raphe nucleus (DRN) does not affect seizure duration or severity. (A,B) Seizure-induced mortality (A) and seizure duration (B) from seizures induced following perfusion of normal (pH 7.4) or acidified artificial cerebrospinal fluid (aCSF; pH 6.8) into the DRN. (C) Seizure duration in mice that survived vs died from the MES seizure. (D) Seizure severity (E/F ratio) following pre-seizure perfusion of acidified aCSF. (E) Seizure severity in mice that survived vs died from the MES seizure. (F) Nissl stained coronal section demonstrating cannula targeted toward the DRN. Aq, aqueduct; DRN, dorsal raphe nucleus; MLF, medial longitudinal fasciculus; scale bar: 2.5 mm.