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. 2021 Dec 23;10(12):bio058976. doi: 10.1242/bio.058976

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Expression of bHLH factors in the AIY lineage. (A) Expression of the NGN-1 protein tagged with GFP (nIs394) in embryos at epidermal enclosure stage before or after the terminal division of the SMDD/AIY mother cell. The AIY lineage is identified with hlh-16p::mcherry (stIs10546). Ventral view, anterior is left, scale bar: 10 µm. (B) Pictures: expression in early postmitotic SMDD and AIY neurons of the NGN-1 protein tagged with GFP (nIs394), HLH-3 protein tagged with mNeonGreen (vlc28), HLH-16 protein tagged with GFP (otEx4503), and HLH-2 protein tagged with GFP (nIs407). The SMDD and AIY neurons are identified with ttx-3pB::mcherry (otIs181 or otEx2644). Epidermal enclosure stage, ventral view, anterior is left, scale bar: 2 µm. Graph: percentage of lineages with a higher level of NGN-1 protein in SMDD than AIY (SMDD>AIY), similar level (SMDD=AIY) or lower level (SMDD<AIY). Error bars show standard error of proportion; n=44 lineages analyzed.