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. 2021 Dec 23;10(12):bio058976. doi: 10.1242/bio.058976

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Effect of combinations of bHLH mutants on AIY neurons at larval stage. Percentage of animals with a loss of ttx-3pB::gfp (otIs173) expression in one or both AIY neurons at late larval stage (L4) in wild type or combinations of ngn-1(ok2200), hlh-16(ot711) and hlh-3(tm1688) mutants. Error bars show standard error of proportion; numbers below genotypes show numbers of animals analyzed. Statistics: each mutant genotype is different from wild type (***P<0.001), in addition, each double mutant is different from the corresponding single mutants (P<0.01, enhancement for ngn-1; hlh-16 and hlh-16; hlh-3, suppression for ngn-1; hlh-3), the triple mutant is different from the double mutants (P<0.001, enhancement), Fisher's exact test with Bonferroni correction for multiple comparisons.