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. 2021 Dec 23;10(12):bio058976. doi: 10.1242/bio.058976

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — bHLH binding sites in the ttx-3 and ceh-10 cis-regulatory regions. (A) Sequence of the cis-regulatory region that regulates the initiation of ttx-3 expression in the AIY lineage. (B) Region of the ceh-10 locus sufficient to rescue a ceh-10 mutant. ceh-10 is located in introns of another gene (polq-1) in the opposite direction. *: bHLH binding site conserved between C. elegans (C. ele) and at least another species among C. briggsae (C. bri), C. remanei (C. rem) and C. brenneri (C. bre). (C) Quantification of the fluorescence levels of ceh-10endo::yfp (vba1) wild type or mutated for the conserved CATGTG E-box (vba28). Each grey dot represents one neuron. Red bars represent mean and s.d., n=130 neurons analyzed for each genotype, Mann–Whitney test (*P<0.05).