BALF samples were obtained from mice on day 35 post HDM challenge. Levels of BALF cytokines were quantified by multiplex MSD electro-chemiluminescence (OSU core facility). Day 0 represents serum obtained from naïve animals, while day 35 represents serum obtained from animals exposed to HDM for 35 days. KC (A), TNFα (B), IFNγ (C), IL-33 (D), IL-4 level (E), IL-5 (F), IL-17A (G), IL-10 level (H). Data represent the mean ± SD (n = 4) per group obtained from the second in vivo experiment. Multiple t tests performed for statistical analysis, * P < 0.05, ** p < 0.01.