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. 2021 Dec 3;148(23):dev200182. doi: 10.1242/dev.200182

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Identification of the genomic regions enriched in SMAD4-chromatin complexes in mouse forelimb buds. (A) Histogram showing the distribution of SMAD4-interacting regions in relation to the nearest transcriptional start site (TSS) at E9.5-E10.0 (25-30 somites) and E10.5 (34-38 somites). (B) The average Phastcons conservation of the genomic regions enriched in SMAD4-chromatin complexes is shown at both stages. (C) Hierarchical clustering of the high-affinity matches for the top known and de novo motifs enriched in the SMAD4-bound regions at both stages. (D) The top five de novo motif identified in the genomic regions enriched in SMAD4-chromatin complexes in forelimb buds at both stages. (E) ChIP-qPCR validation of two previously known SMAD4-interacting genomic regions – BREs for Id1 and Msx2, respectively – at both stages. Two biological replicates were analyzed (data are mean±s.d. of three technical replicates).