Fig. 2.

Effects of proteotoxic stress on the HB–LATS–AmotL2–YAP complex. (A) Effects of proteasome inhibition on the association between LATS1–Bag3 and AmotL2–Bag3 (the interacting proteins are indicated underneath each set) measured by the proximity ligation assay (see Materials and Methods). MCF10A cells were treated with 5 µM MG132 for the indicated time periods, cells were fixed, and the interactions measured. (B) Effects of proteasome inhibition on association between AmotL2 and YAP. (C) Effects of Bag3 depletion on association between AmotL2 and YAP. *P≤0.05; **P≤0.01 (unpaired two-tailed t-test).