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. 2021 Dec 2;134(23):jcs259107. doi: 10.1242/jcs.259107

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© 2021. Published by The Company of Biologists Ltd

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Fig. 4. — Correlation between C/N ratio of YAP and C/N ratio of Bag3 in cell population. Cells expressing FLAG-tagged Bag3 were double stained for FLAG and YAP. (A) The C/N ratio (cytoplasmic/nuclear localization) of YAP in each cell was plotted against the C/N ratio of Bag3 in the same cell. In control sample there is no correlation between C/N ratios of Bag3 and YAP in individual cells. On the other hand, upon MG132 treatment, the correlation between nuclear distribution of YAP and Bag3 becomes obvious. (B) Effects of MG132 on C/N distribution of YAP and Bag3. In A, we performed single linear regression using a Person's R with a confidence level of 95% and then calculated R-squared.