Skip to main content
. 2021 Dec 2;134(23):jcs259107. doi: 10.1242/jcs.259107

Fig. 7.

Fig. 7.

Distribution of cells in the population according to C/N ratio of YAP and Bag3 in cells seeded at low and high density. Low density corresponds to 3000 cells/well and high density to 19,500 cells/well. (A) Representative image of localization of YAP in low and high density cells in the presence and absence of MG132. Arrows indicate nuclei with high content of either YAP or Bag3. Circles indicate nuclei with low content of YAP or Bag3. (B) Representative image of localization of Bag3 in low and high density cells in the presence and absence of MG132. Arrows indicate nuclei with high content of either Bag3. Circles indicate nuclei with low content of Bag3. (C) YAP C/N ratio (cytoplasmic/nuclear localization) distribution in the population at low and high density. Images in A and B are representative of three experiments. (D) Bag3 C/N ratio distribution in the population at low and high density. E. Distribution of cells in the population according to C/N ratio of YAP upon depletion of Bag3. Effect of depletion of endogenous Bag3 on intracellular distribution of YAP at low and high density. A control for Yap expression in different cell densities and in the absence of Bag3 using siRNA Bag3 is shown in Fig. S4. *P≤0.05; **P≤0.01; ***P≤0.001; ****P≤0.0001  (unpaired two-tailed t-test).