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. 2021 Dec 10;13(23):25241–25255. doi: 10.18632/aging.203742

Figure 5.

Figure 5

miR-106a-5p inhibits Foxo1 expression by targeting its 3’UTR. (A) Putative miR-106a-5p binding sequences in wild-type or mutated Foxo1 3’UTR. (B) Luciferase report assay showed the direct relationship between wild-type Foxo1 3’UTR and miR-106a-5p (**p < 0.01 miR-106a-5p vs. mimics NC, **p < 0.01 miR-106a-5p inhibitor vs. inhibitor NC). (C) Luciferase report assay showed the direct relationship between Foxo1 3’UTR mutation and miR-106a-5p. (D) qRT-PCR, (E) western blot analysis, and (F) IF staining detected Foxo1 mRNA and protein levels in VSMCs overexpressing miR-106a-5p (*p < 0.05 miR-106a-5p vs. mimics NC). (G) qRT-PCR, (H) western blot analysis, and (I) IF staining detected Foxo1 mRNA and protein levels in VSMCs incubated with exosomes (**p < 0.01 ECs-Exo-NG vs. ECs-Exo-HG, **p < 0.01 NC-Exo vs. circHIPK3-Exo).