Figure 2.

AhR KO suppresses IL22-induced colonic cell proliferation in vivo. Expression of Reg3b in sorted colonic stem cells (A) and sorted colonic progenitor cells (B), n = 6–8 mice per group. C: representative images of cell proliferation marked by EdU⁺ in the distal colon of n = 3 or 4 mice treated with IL22 or vehicle (control) for 2 days. GFP⁺ crypts were quantified; green, GFP; blue, DAPI; white, EdU. Scale bar 50 μm. Quantification of EdU⁺ cells (D) or EdU⁺ stem cells (E) per crypt. n = 64–102 from n = 3 or 4 mice. Data were analyzed using a one-way ANOVA with Tukey’s multiple-comparisons test in D and E. *P < 0.05, ***P < 0.001, ****P < 0.0001. AhR, aryl hydrocarbon receptor; GFP, green fluorescent protein; KO, knockout; WT, wild type.