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. 2021 Nov 10;322(1):G93–G106. doi: 10.1152/ajpgi.00074.2021

Figure 4.

Figure 4.

SOCS3 expression is upregulated in AhR-deficient organoids. mRNA expression of Socs3 in sorted GFPhi colonic stem cells (A), sorted GFPlow colonic progenitor cells (B), and colonic organoids treated with DMSO or 10 nM TCDD (C), n = 4 mice per group. D: representative immunoblots for SOCS3 in AhR WT and KO colonic organoids treated with DMSO or TCDD, n = 3 mice per group. E: quantification of SOCS3 protein level. Data were analyzed using a paired Student’s t test within the same genotype. F: representative images of SOCS3 expression in colonic crypts. GFP+ crypts were quantified; magenta, SOCS3; green, GFP; blue, DAPI. Scale bar 50 μm. G: quantification of mean fluorescence intensity of SOCS3 per crypt, n = 3 mice per group. *P < 0.05, **P < 0.01, ****P < 0.0001. AhR, aryl hydrocarbon receptor; GFP, green fluorescent protein; KO, knockout; SOCS3, suppressor of cytokine signaling 3; TCDD, tetrachlorodibenzodioxin; WT, wild type.