Figure 4.

The expression of miRNA-185-3p that the barrier function in CaCo₂ monolayer cells regulating by HQT. The model group was treated with TNF-α for 24 hours after CaCo₂ cells grew into a monolayer, and the HQT groups were treated with TNF-α for 2 hours. Then, 50 µg/mL of HQT was added to the CaCo₂ cells. After 24 hours, the TEER value was observed (a). The expression of MLCK and occludin protein was tested by WB (b–d). The expression of MLCK mRNA (e) was tested by q-PCR. Mimic NC, mimic miRNA-185-3p (mi), inhibitor NC, and inhibitor miRNA-185-3p (in) were transinfected, respectively, by CaCo₂ cells. After adding HQT into miRNA-185-3p (f), the change of the TEER value (g) and the change of MLCK/p-MLC (h, i, j), their expression were tested (n = 3, ^∗P < 0.05, ^∗∗P < 0.01; ^#P < 0.05, ^##P < 0.01). β-actin.