Figure 4.

GLY and anti-HMGB1 antibody alleviated the GEC injury in LN. (a–d) TEER and permeability of HRGEC in control, LN, LN+GLY, LN+H ab, and LN+IgG ab groups (n = 5). (e, f) SDC-1 (green) in HRGECs was detected by IF staining. (g, h) The level of SDC-1 in supernatant was measured by ELISA (n = 5). (i, j) Colocalization of VE-cadherin (green) and F-actin (red) in HRGECs was detected by IF staining. (k, m) The expression of VCAM-1 in HRGECs was measured by western blotting. (l, n) The level of NO in HRGECs (n = 5). GLY: glycyrrhizin; H ab: anti-HMGB1 antibody; IgG ab: control IgG.