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. 2021 Jun 7;4(6):e1417. doi: 10.1002/cnr2.1417

FIGURE 3.

FIGURE 3

TDO2 promotes cell proliferation in BC cells. (A) TDO2 expression in seven BC cell lines by qRT‐PCR analysis. The data are displayed as mean ± SD, (n = 3). (B) Western blot analysis shows that 253‐JBV and UMUC6 cells highly expressed TDO2 among seven BC cell lines. (C) TDO2 mRNA levels expression in 253‐JBV cells transfected with two siRNAs targeting TDO2 (siRNA1, siRNA2) and negative control (NC) siRNA by qRT‐PCR analysis. The data are displayed as mean ± SD, (n = 3). (D) The proliferation of TDO2 siRNA‐transfected 253‐JBV cells was evaluated at 1, 2, and 4 days. NC: negative control. The error bars indicate SE, (n = 3). (E) TDO2 mRNA levels expression in RT112 cells transfected with TDO2 expression vector (pDON5‐TDO2) and empty vector by qRT‐PCR analysis. The data are displayed as mean ± SD, (n = 3). (F) The proliferation of RT112 transfection with TDO2 expression vector or empty vector was evaluated at 1, 2, and 4 days. The error bars indicate SE, (n = 3). *p < .01