FIGURE 6.

Effect of TDO2 on cetuximab sensitivity in BC cells. (A) Western blot analysis of TDO2, Erk1/2, phospho‐Erk1/2 (pErk1/2), Akt, and phospho‐Akt (pAkt) in 253‐JBV cells transfected with TDO2 siRNAs (siRNA1, siRNA2) or negative control siRNA (NC). β‐Actin was served as a loading control. (B) Western blot analysis of TDO2, Erk1/2, pErk1/2, Akt, and pAkt in RT112 cells transfected with TDO2 expression vector (pDON5‐TDO2) or empty vector. β‐Actin was served as a loading control. (C) Dose‐dependent effect of cetuximab on the viability of 253‐JBV cells transfected with TDO2 siRNAs or negative control siRNA. (D) Dose‐dependent effect of cetuximab on the viability of UMUC6 cells transfected with TDO2 siRNAs or negative control siRNA. The error bars indicate SE, (n = 3). *p < .01