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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2021 Sep 15;16(4):796–805. doi: 10.1007/s11481-021-10011-w

Figure 2. Donor hu-HSC mice.

Figure 2.

The dynamics of (A) plasma viral load (VL) and (B) peripheral blood human CD4+ T cells in individual donor hu-HSC mice were recorded. The detection limit (DL) for plasma HIV-1 RNA capture was below 200 copies/ml as shown by the dotted line. Paraffin-embedded brain sections were collected and subjected to (C) immunohistochemistry staining for human HLA-DR as well as (D) RNAscope analysis for HIV-1 RNA detection as shown by a single or clusters of brown dots. (E) Ultrasensitive ddPCR, with sensitivity of detecting 1–2 viral copies, was used in cross validation tests for viral DNA detection from brains.