FIG. 12.

Cell death induced by overexpression of ΔN ASK1 is partially suppressed by dominant-negative (d/n) forms of MLK3 and DLK. pCMS.EGFP (GFP) and pCMS.EGFP-ΔNASK1 (ΔN ASK1) were cotransfected with either pCDNA3 vector or constructs encoding dominant-negative MLK3 or dominant-negative DLK (in the pCDNA3 vector) as described in Materials and Methods. Three days after transfection, the proportions of apoptotic nuclei were assessed as described in Materials and Methods. Because the results for pCMS.EGFP-pCDNA3 and pCMS.EGFP cotransfected with dominant-negative MLK3 or dominant-negative DLK were not apparently different from one other, only the results for pCMS.EGFP-pCDNA3 are shown. The values are the means from three independent experiments plus SEM.